ID:
989
Typing of DRB1, DQB1, DQA1 class II HLA genes
Method:
FLUORESCENCE Endpoint detection
Full name:
HLA II DRB1, DQB1, DQA1 Typing of class II HLA genes - 3 genes
Sample Type:
Sample quantity:
Sample container:
Blood
5 ml
A tube with a purple cap
Typing of Class II HLA Genes: HLA-DRB1, HLA-DQB1, HLA-DQA1
The second class HLA (Human Leukocyte Antigen) genes are a group of genes that play an important role in the functioning of the immune system. These genes are involved in the regulation of the immune response and the recognition of foreign antigens entering the body. HLA gene typing is important in the study of transplantation, diagnosis of autoimmune diseases and some infectious diseases. Three genes are mainly considered in this typing process - HLA-DRB1, HLA-DQB1, and HLA-DQA1 .
HLA-DRB1
Function : HLA-DRB1 gene encodes the beta chain of HLA-DR molecules. HLA-DR molecules are located on the surface of antigen-presenting cells and present antigens to CD4+ T cells.
Clinical Significance : HLA-DRB1 alleles are associated with various autoimmune diseases, such as rheumatoid arthritis, type 1 diabetes, and multiple sclerosis.
HLA-DQB1
Function : HLA-DQB1 gene encodes the beta chain of HLA-DQ molecules. HLA-DQ molecules are involved in the process of presenting antigens.
Clinical Significance : HLA-DQB1 variations are associated with diseases such as celiac disease, type 1 diabetes and narcolepsy.
HLA-DQA1
Function : HLA-DQA1 gene encodes the alpha chain of HLA-DQ molecules. These molecules play an important role in regulating the immune response.
Clinical Significance : HLA-DQA1 alleles can be identified as risk factors for autoimmune diseases, associated with diseases such as celiac disease and lupus.
HLA Gene Typing Process
Typing of HLA genes is carried out by different methods:
PCR (Polymerase Chain Reaction) : This method amplifies specific HLA genes.
Sequence Analysis : This method is used to determine the exact sequence of genes.
SSP (Sequence-Specific Primers) : This method uses specific primers for precise determination of HLA alleles.
NGS (Next-Generation Sequencing) : This method is used to collect genetic information on a large scale and enables high-precision typing.
Areas of Application
Transplantation : HLA typing is important to determine donor and recipient compatibility in organ transplantation.
Autoimmune Diseases : Typing is used to determine the risk of autoimmune diseases associated with HLA genes.
Pharmacogenomics : HLA genetic typing can be used to predict the efficacy or toxicity of certain drugs.
In general, typing of HLA-DRB1, HLA-DQB1, and HLA-DQA1 genes has an important role in medical research and clinical diagnosis and helps to develop individualized treatment approaches.
DRB1 :
Rheumatoid arthritis (RA) : Joint pain, swelling, and stiffness.
Multiple Sclerosis (MS) : Vision problems, muscle weakness, gait and balance problems.
Type 1 Diabetes (T1D) : Excessive thirst, frequent urination, weight loss.
DQB1 :
Celiac disease : Abdominal pain, diarrhea, weight loss, iron deficiency anemia.
Narcolepsy : Excessive sleepiness, cataplexy (sudden muscle weakness caused by overwhelming emotions).
Type 1 Diabetes : Same as DRB1, symptoms of T1D.
DQA1 :
Celiac disease : Similar symptoms to DQB1, as both genes play an important role in this disease.
Autoimmune Thyroid Disease (AITD) : Fatigue, weight changes, sensitivity to heat and cold.